Cell Counting Kit-8 (CCK-8)

产品优势
实验数据
价格对比
产品描述
实验方法
储存条件
资料下载

产品优势

检测方法	CCK-8 (WST®-8)	WST-1	XTT/MTS	MTT
灵敏度	+++++	+++	+++	+
检测耗时	0.75h	0.75h	2.5h	3.75h
便捷程度	+++++	+++	+++	+

实验数据

Product Use Citation(55)

Molecules, 2019, 24(1)
PubMed: 30577522
Cells, 2019, 8(4)
PubMed: 30935108
Molecules, 2019, 24(7)
PubMed: 30979064
Cancer Lett, 2019, 447:1-11
PubMed: 30673593
Lab Invest, 2019, 10.1038/s41374-019-0249-2
PubMed: 30988371
Cancer Med, 2019, 8(2):656-668
PubMed: 30636383
Oncol Rep, 2019, 41(3):1487-1496
PubMed: 30592293
Kaohsiung J Med Sci, 2019, 10.1002/kjm2.12058
PubMed: 30903681
J Exp Clin Cancer Res, 2019, 38(1):4
PubMed: 30611309
Int J Mol Sci, 2019, 20(4)
PubMed: 30781441
J Exp Clin Cancer Res, 2018, 37(1):263
PubMed: 30376856
EBioMedicine, 2018, 38:89-99
PubMed: 30470613
Cell Death Dis, 2018, 9(12):1146
PubMed: 30451837
Cell Physiol Biochem, 2018, 51(4):1616-1631
PubMed: 30497065
Int J Biol Sci, 2019, 15(2):430-440
http://www.ijbs.com/v15p0430.htm
Cancer Manag Res, 2018, 10:5785-5798
PubMed: 30510448
Toxicol Appl Pharmacol, 2019, 363:64-71
PubMed: 30476502
Mol Cell Endocrinol, 2018, 478:53-61
PubMed: 30025915
Acta Pharmacol Sin, 2018, 10.1038/s41401-018-0165-9
PubMed: 30315250
Molecules, 2018, 10.3390/molecules24010011
PubMed: 30577522
Oncol Rep, 2018, 10.3892/or.2018.6951
PubMed: 30592293
Int J Mol Med, 2018, 42(6):3424-3436
PubMed: 30320379
Onco Targets Ther, 2018, 11:6579-6587
PubMed: 30323630
Biochem Biophys Res Commun, 2018, 500(3):650-657
PubMed: 29678569
FEBS Lett, 2018, 592(18):3092-3100
PubMed: 30112836
Atherosclerosis, 2018, 274:128-138
PubMed: 29772481
Cancer Sci, 2018, 109(4):1220-1229
PubMed: 29453896
J Cell Physiol, 2018, 233(6):4864-4884
PubMed: 29334122
Mol Cancer Res, 2018, 16(3):517-527
PubMed: 29330283
Biochem Biophys Res Commun, 2018, 498(3):495-501
PubMed: 29526753
Biochem Biophys Res Commun, 2018, 495(2):1908-1914
PubMed: 29242154
Oncol Rep, 2018, 39(5):2071-2080
PubMed: 29565449
Br Poult Sci, 2018, 10.1080/00071668.2018.1426832
PubMed: 29355473
Int J Oncol, 2018, 53(3):1138-1148
PubMed: 29956730
Cancer Cell Int, 2018, 18:104
PubMed: 30038550
Pharmacol Res, 2018, 129:432-442
PubMed: 29154988
Mol Vis, 2018, 24:509-517
PubMed: 30090014
Mol Carcinog, 2018, 57(8):1067-1077
PubMed: 29667779
Exp Ther Med, 2018, 15(6):4885-4889
PubMed: 29805510
Exp Ther Med, 2018, 15(6):5269-5274
PubMed: 29805545
Cell Prolif, 2018, 51(3):e12447
PubMed: 29484736
Bioorg Med Chem, 2018, 26(14):4179-4186
PubMed: 30006143
Arch Biochem Biophys, 2018, 10.1016/j.abb.2018.09.007
PubMed: 30222953
Acta Pharmacol Sin, 2018, 39(4):642-648
PubMed: 29239350
Mediators Inflamm, 2018, 2018:9787239
PubMed: 29736154
Mol Med Rep, 2018, 17(6):7618-7626
PubMed: 29620262
Cryobiology, 2018, 10.1016/j.cryobiol
PubMed: 29885288
Exp Cell Res, 2018, 362(2):436-443
PubMed: 29233682
J Cell Physiol, 2018, 233(4):3066-3079
PubMed: 28681913
Am J Transl Res, 2017, 9(5):2567-2578
PubMed: 28560005
Oncotarget, 2017, 8(15):24840-24852
PubMed: 28193910
Cancer Sci, 2017, 108(2):267-276
PubMed: 27906498
Pharmacol Res, 2017, 119:137-148
PubMed: 28167239
IUBMB Life, 2016, 68(9):764-75
PubMed: 27519168
Nanoscale, 2016, 8(44):18800-18807
PubMed: 27805234

Customer Product Validation(10)

MCF-7, T47D or MDA-MB-231 cells were treated with a series of concentrations of RA for 48 h in the absence or presence of 20 μmol/L CQ. After treatment, cell viability was measured using a CCK8 reagent. The results are displayed as the mean±SD of triplicate measurements from one of three identical experiments; *P<0.05, **P<0.01, t-test, RA vs RA+CQ.
The effects of compound on the cell viability of NAMALWA cells.
Survival rate of post thaw E. akaara sperm in different concentrations of glycerol and dimethyl sulfoxide (DMSO) as cryoprotective agents (CPAs). Different superscripts (normal font for glycerol, italic font for DMSO) indicate significant differences between concentrations in each CPA (P < 0.05). Asterisks indicate significant differences between glycerol and DMSO at that concentration (P < 0.05). The post-thaw spermsurvival rate was determined using a Cell Counting Kit-8 from Bimake.
Cellular proliferation quantified by cell counting using Cell Counting Kit-8 assay.
Cell were treated with si-RNA of SORCIN and/or ADR and cell viability was detected by CCK-8. Columns, values are expressed as mean ± SD. **p < 0.01, ***p < 0.001 versus that obtained in the control group.
Cell viability of different groups measured using a Cell Counting Kit-8 kit (n=3).
Detection of the proliferation of H522 cells transfected with siCtrl, siTMEM100-1 or siTMEM100-2 by CCK8 assay.
CCK8 assays were performed to inspect the growth of pancreatic cells after alteration in LDHA expression.
Relative expression of ST3GAL6 was detected by quantitative real-time PCR and Western blot analysis in the two hepatoma cell lines after transfection. Proliferation curves according to the results of CCK-8 assay (b)
Fig. Different amount of cells were seeded into 96-well plate at different concentrations in 100 μl culture medium in triplicate. After adherence, 10 μl/well Biotool Cell Counting Kit-8 (CCK-8) or Dojindo CCK-8 was added, allowing cells to continuously culture for indicated duration in cell culture incubator. Absorbance at 450 nm was then measured in a microplate reader. This data demonstrates comparable performance of Biotool Cell Counting Kit-8 (CCK-8) and Dojindo CCK-8.

价格对比

Cell Counting Kit-8 (CCK-8) | Price Comparison

Brand	Price/Reaction
Bimake	0.2994 RMB
Dojindo	0.7440 RMB
Sigma	3.2208 RMB

产品描述

Bimake Cell Counting Kit-8 (CCK-8)是一种氧化还原反应的指示剂，在电子载体1-Methoxy PMS存在的条件下，利用活细胞中的脱氢酶催化四唑盐WST®-8生成甲臜染料（图1），而且甲臜染料的生成量与活细胞的数量成线性关系（图2）。该试剂的灵敏度高于MTT、XTT、MTS或WST-1等其他四唑盐。
Bimake Cell Counting Kit-8 (CCK-8)细胞活性检测试剂为一瓶即开即用的溶液，该试剂安全，无放射性，性价比高，为客户提供方便、快速、可靠的使用体验。

图1: Working mechanisms of Cell Counting Kit-8 (CCK-8).

图2: 使用Bimake Cell Counting Kit-8 (CCK-8)进行细胞计数和OD值的比较。按不同的密度将K562和Hela细胞接种在96孔板的100 μl培养基中（每个样品进行三个重复）. 培养16h后，在孔中添加10 μl Cell Counting Kit-8 (CCK-8)，并在培养箱按指定时间进行孵育。用培养基作为空白对照，在酶标仪450 nm下检测光吸收值。

图3: 使用Bimake Cell Counting Kit-8 (CCK-8)进行细胞计数和OD值的比较。按不同的密度将HEK293细胞接种在96孔板的100 μl培养基中（每个样品进行三个重复）. 培养16h后，在孔中添加10 μl Cell Counting Kit-8 (CCK-8)，并在培养箱按指定时间进行孵育。用培养基作为空白对照，在酶标仪450 nm下检测光吸收值。

实验方法

需要的实验器材

• 酶标仪
• 多道移液器
• 3 % SDS（可选）

实验步骤

1) 接种细胞于培养瓶并使之生长4–24小时，然后开始检测。
2) 在细胞培养液中直接加入1/10体积的Bimake Cell Counting Kit-8 (CCK-8)，充分混合，保证孔中颜色均一性，但不可有气泡产生。对96孔板，每100µl培养液加入10µl活性检测试剂。
3) 继续培养1-4小时至颜色变为橙色，如细胞密度/活力过低，可适当延长培养时间至24小时，但需注意不可孵育过度。
4) 用酶标仪读取450nm光吸收值。典型的OD值在0.8-1.5之间，但0.5-2.5都是可接受的范围。
注意：在添加Cell Counting Kit-8 (CCK-8)的细胞培养基中，在450nm处会有轻微的自发吸收值。该荧光背景随培养基、pH值、培养时间、暴露于光线的时间等因素的变化而变化。典型的吸收值在孵育2小时后为0.1-0.2。为了纠正背景，准备一个或多个无细胞的对照孔，计算其平均的荧光值，然后从试验孔中扣除该值。
5) 可选步骤：在100μl细胞中加入10 μl 3 % SDS 以终止反应，可在3天内读取数据。
6) 细胞活力计算：
细胞活力*（％）=[A（药物+）— A（空白）] / [A（药物—）— A（空白）] ×100%
A（药物+）：具有细胞、CCK-8溶液和药物溶液的孔的吸光度；
A（药物—）：具有细胞、CCK-8溶液而没有药物溶液的孔的吸光度；
A（空白）：具有培养基和CCK-8溶液而没有细胞的孔的吸光度。
*细胞活力：细胞增殖活力或细胞毒性活力

实验简要流程

应用领域

1) 细胞增殖测定——Bimake Cell Counting Kit-8 (CCK-8)具有水溶性、稳定性好、无细胞毒性。
2) 细胞活力检测——细胞代谢活力与橙色的产生随细胞活力的变化而变化。
3) 细胞因子实验——测定细胞因子诱导的细胞增殖，克隆形成及生长。
4) 细胞毒性实验——有毒的化学物质会导致细胞死亡，但死亡的细胞不会还原WST8，只有剩余的活细胞能够显色。Bimake Cell Counting Kit-8 (CCK-8)本身对细胞无毒性。

注意事项

1. 使用前请充分混匀，避免反复冻融。
2. 如果长时间孵育（过夜），请确保在试剂添加与孵育期间维持无菌状态。细菌也能够是本试剂显色。
3. 使用本品必须包含阳性、阴性和无细胞（纯培养液）对照。
4. 本产品只能用于研究，不能用于药物、日常生活等其他方面。

储存条件

Bimake Cell Counting Kit-8 (CCK-8)在避光条件下可在室温保存6个月，2-8°C保存2年，-20°C及以下可保存更长时间。

资料下载

Cell Counting Kit-8 (CCK-8)

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产品类别

Cell Counting Kit-8 (CCK-8)

产品优势

实验数据

Product Use Citation(55)

Customer Product Validation(10)

价格对比

产品描述

实验方法

需要的实验器材

实验步骤

实验简要流程

应用领域

注意事项

储存条件

资料下载

技术支持