qRT-PCR assay of mRNA level of SOSTDC1 in clinical samples. SYBR Green qPCR Master Mix is purchased from Bimake.

(C-J) qRT-PCR assay was performed to detect changes in mRNA expression of some genes involved in NF-κB signaling pathway after transfection of DPCR1 shRNA plasmids in PANC-1 and MIA PaCa2 cells. SYBR Green qPCR Master Mix is purchased from Bimake.

Solanine A suppressed the mRNA expression of pro-inflammatory cytokines and chemokines in LPS/IFNγ-stimulated RAW264.7 cells. Cells were pre-treatedwith different concentrations of solanine A and BAY 11-7082 (5 μM) for 2 h, and then stimulated with LPS/IFNγ (0.5 μg/mL and 20 ng/mL) for 24 h. (A) The mRNA levels of TNF-α (A), IL-1β (B), IL-6 (C) and CXCL9 (D) were measured by qRT-PCR, GAPDH was used as an internal control. ** p < 0.01, *** p < 0.001 vs. LPS/IFNγalone, n = 3.

The mRNA expression level of TTP in lung adenocarcinoma cells by qRT-PCR. The SYBR Green qPCR Master Mix is purchased from Bimake.

D, qRT-PCR analysis showed that XIST could negatively regulate miR-195-5p expressionin OS cells. E, Dual-luciferase reporter assays showed that miR-195-5p could negatively regulate the luciferase activity of XIST-WT but not XIST-MUT. F-G, XIST was enriched in the Ago2 immunoprecipitates relative to the control IgG immunoprecipitates. H, miR-195-5p inversely regulates XIST expression in OS cells. (*P < 0.05)

(A-C) qPCR analysis of mRNA isolated from (A) MDA-MB-231, (B) U87, or (C) HT1080 cells. Graphs show relative expression of LPAR1, LPAR2, LPAR3 and LPAR4 mRNA in copy number. RPLP0 was used to normalize the data (n = 3 independent experiments).

(c) HBE16 cells were pretreated with APS (200 μg/ml) for indicated times. The mRNA expression of LL-37 was measured by RT-qPCR. The LPS (1 μ g/ml) group was used as the positive control. SYBR Green qPCR Master Mix is purchased from Bimake.

(A-I) Comparison of cell death‐related gene expression among UC3 cells with control (ctrl), melatonin (mel), VPA and combinatorial (comb) treatment by qPCR. The data are presented as mean ± SD, n = 3. *P < 0.05 vs. control, **P ≤ 0.01 vs. control, and ***P ≤ 0.001 vs. control.

RT-qPCR analysis of HEK293 and HeLa cells transfected with a control, PKV VP3 or L for 24 h followed by IFN-β or IFN-γ (50 ng/ml) treatment for 0-8 h

Western blot analysis of whole-cell lysates from differentiated C2C12 cells (b) with indicated antibodies. qRT-PCR analysis of differentiated C2C12 cells © with FTO. Asterisks indicate statistical significance (*P<0.05, **P<0.01, ***P<0.001)