q-PCR analyses of MTDH expression levels in the GC MKN45, BGC823, HGC803, AGS, and SGC7901 cell lines and the normal gastric mucosal GES-1 cell line. q-PCR was performed using SYBR Green I Master Mix (Bimake).
qRT-PCR assay of mRNA level of SOSTDC1 in clinical samples. SYBR Green qPCR Master Mix is purchased from Bimake.
(C-J) qRT-PCR assay was performed to detect changes in mRNA expression of some genes involved in NF-κB signaling pathway after transfection of DPCR1 shRNA plasmids in PANC-1 and MIA PaCa2 cells. SYBR Green qPCR Master Mix is purchased from Bimake.
Solanine A suppressed the mRNA expression of pro-inflammatory cytokines and chemokines in LPS/IFNγ-stimulated RAW264.7 cells. Cells were pre-treatedwith different concentrations of solanine A and BAY 11-7082 (5 μM) for 2 h, and then stimulated with LPS/IFNγ (0.5 μg/mL and 20 ng/mL) for 24 h. (A) The mRNA levels of TNF-α (A), IL-1β (B), IL-6 (C) and CXCL9 (D) were measured by qRT-PCR, GAPDH was used as an internal control. ** p < 0.01, *** p < 0.001 vs. LPS/IFNγalone, n = 3.
The mRNA expression level of TTP in lung adenocarcinoma cells by qRT-PCR. The SYBR Green qPCR Master Mix is purchased from Bimake.
D, qRT-PCR analysis showed that XIST could negatively regulate miR-195-5p expressionin OS cells. E, Dual-luciferase reporter assays showed that miR-195-5p could negatively regulate the luciferase activity of XIST-WT but not XIST-MUT. F-G, XIST was enriched in the Ago2 immunoprecipitates relative to the control IgG immunoprecipitates. H, miR-195-5p inversely regulates XIST expression in OS cells. (*P < 0.05)
(A-C) qPCR analysis of mRNA isolated from (A) MDA-MB-231, (B) U87, or (C) HT1080 cells. Graphs show relative expression of LPAR1, LPAR2, LPAR3 and LPAR4 mRNA in copy number. RPLP0 was used to normalize the data (n = 3 independent experiments).
(c) HBE16 cells were pretreated with APS (200 μg/ml) for indicated times. The mRNA expression of LL-37 was measured by RT-qPCR. The LPS (1 μ g/ml) group was used as the positive control. SYBR Green qPCR Master Mix is purchased from Bimake.
(A-I) Comparison of cell death‐related gene expression among UC3 cells with control (ctrl), melatonin (mel), VPA and combinatorial (comb) treatment by qPCR. The data are presented as mean ± SD, n = 3. *P < 0.05 vs. control, **P ≤ 0.01 vs. control, and ***P ≤ 0.001 vs. control.
RT-qPCR analysis of HEK293 and HeLa cells transfected with a control, PKV VP3 or L for 24 h followed by IFN-β or IFN-γ (50 ng/ml) treatment for 0-8 h
Bimake 2x SYBR Green qPCR master mix是使用SYBR Green I嵌合荧光法进行定量PCR的专用试剂。产品是一种2×预混合试剂,包括一种特殊的热启动Taq DNA Polymerase和qPCR反应的最佳Buffer的体系,可以有效抑制非特异扩增,从而显著提高扩增效率和检测灵敏度。由于采用热启动酶的激活机制,可以在室温条件操作,实验结果精确性高,重复性好,可信度强。
产品应用
基于荧光定量PCR检测 核酸扩增和基因表达 自动化和高通量的定量基因分型研究 基因组学相关应用
产品组成
Component
Cat #: B21202
Cat #: B21203
Bimake™ 2x SYBR Green qPCR Master Mix※
5 ml (for 200 reactions with 50 μl/rxn for 500 reactions with 20 μl/rxn for 1000 reactions with 10 μl/rxn)
25 ml (for 1000 reactions with 50 μl/rxn for 2500 reactions with 20 μl/rxn for 5000 reactions with 10 μl/rxn)