首页 > 蛋白纯化和免疫沉淀的磁珠系列 > Protein A/G免疫沉淀磁珠

Protein A/G免疫沉淀磁珠

产品号

规格

单价

数量

B23201	1 mL	RMB 438.76
B23202	5 mL	RMB 1800.08

加入购物车联系我们

今日订购，明日送达，全国免运费！

订单及询单:电话: 400-115-0509邮箱: [email protected]

产品优势
实验数据
价格对比
产品描述
储存条件
实验方法
资料下载

产品优势

省时：比亲和凝胶法节省大约80min（以IP实验为例比较）；
操作简单：磁性分离，无需离心；
非特异低：亲和凝胶孔径大，容易产生非特异吸附，而磁珠孔径小，不容易产生非特异吸附；
结合量高：磁珠表面PorteinA/G的蛋白包被量高达9.3×1013个分子/平方厘米，抗体加载量为0.6-0.8 mg/mL磁珠悬液。

实验数据

Customer Product Validation(9)

The ubiquitination level of Mfn1, Mfn2 and Cx43 in cardiomyocytes which were transfected with gp78-siRNA or Con-siRNA and treated by 5 μM NPS R568 or 3 μM Calhex231 respectively by immunoprecipitation (n= 3). A representative blot is shown. *P<0.05 versus control; # P<0.05 versus HG
(F) Whole-cell lysates from osteosarcoma cell lines were immunoprecipitated with an anti-CSE1L antibody followed by immunoblotting (IB) with anti-MSH6 and anti-CSE1L antibodies. IgG was used as a negative control. (G) Whole-cell lysates from osteosarcoma cell lines were immunoprecipitated with an anti-MSH6 antibody followed by IB with anti-CSE1L and anti-MSH6 antibodies. IgG was used as a negative control.
Immunoprecipitation for c-Myc protein:
100 μg of Protein A/G Magnetic beads & 10 μg anti-c-Myc antibody was used.
Immunoprecipitation for Flag Tag-fusion Protein:
1. 1% input
2. Biotool Magnetic Protein A/G beads
3. Biotool Magnetic Protein A/G beads + Mouse Anti-Flag Antibody
4. Life technology Magnetic Protein A/G beads + Mouse Anti-Flag Antibody + IP
5. Biotool Magnetic Protein A/G beads + Mouse Anti-Flag Antibody + IP
6. Santa cruz Protein A/G Agarose gel + Mouse Anti-Flag Antibody + IP
Immunoprecipitation for HAS:
1. 5 ug of rabbit polyclonal antibody was used
2. 5 ug of mouse IgG1 monoclonal antibody was used
Immunoprecipitation for GST-Tag Fusion Protein:
100 ug of Biotool Protein A/G Magnetic beads & 10 ug Anti- GST-Tag Antibody was used
(I and J) 293T cells were co-transfected with a HA-tagged TGEV S1 expression plasmid together with GFP-tagged EGFR Receptor 1 or GFP-tagged EGFR Receptor 2 expression plasmid, cell lysates were immunoprecipitated with an anti-HA antibody or an anti-GFP antibody, the resulting precipitates were examined by immunoblotting using an anti-HA or an anti-GFP antibody to examine the interaction between HA-TGEV S1 and GFP-tagged EGFR. (** p < 0.01).
Immunoprecipitation for HAS:
100 ug of Biotool Protein A/G Magnetic beads & 10 ug Anti-HAS Antibody was used
Immunoprecipitation for GST-Tag Fusion Protein:
1. Antigen & Anti-GST-Tag Antibody (Mouse IgG1)
2 & 3. Elution after IP
4. Antigen & Anti-GST-Tag Antibody (Mouse IgG1)
5 & 6. Elution after IP (Control group without GST-Tag Fusion Protein)

价格对比

产品描述

Protein A/G免疫沉淀磁珠使用“纳米表面生物技术”(S-TEC)，Protein A/G包被在超顺磁性磁珠微球表面，包被密度高达9.3×10¹³个分子/平方厘米。与同类产品相比，Bimake Protein A/G免疫沉淀磁珠表面具有更多的抗体结合位点。在IP实验中，只需使用更少的磁珠量，非特异性结合低，使用简便有效。Bimake Protein A/G免疫沉淀磁珠具有大面积特异的表面区域，从而大大缩短抗体吸附和抗原结合的时间。最短时，抗原和抗体的结合时间只需10分钟，可以在30分钟内完成完整的IP实验。Bimake Protein A/G免疫沉淀磁珠适用的范围广泛，例如细胞裂解液、细胞分泌液上清、血清、动物腹水以及其它的免疫抗原等。

特性

Protein A/G是融合了Protein A和Protein G蛋白的IgG结合区的重组融合蛋白，它包含了Protein A的四个Fc结合区域以及Protein G的两个Fc结合区域，分子量50460 Da。相比Protein A，Protein A/G的结合更少依赖pH值，但是却具有了Protein A和G两者的加合效应。

产品性质

浓度	10 mg/mL
磁珠大小	100 nm
人IgG结合能力	0.4-0.5 mg/mL
配体	重组蛋白A/G
应用	rProtein纯化，免疫沉淀
pH稳定性	6-8 （长期）
运输条件	蓝冰

储存条件

4°C可以储存2年。在缺乏甘油的情况下避免反复冻融。

实验方法

图1展示了免疫共沉淀（IP）的操作方法。可以根据抗体类型和靶蛋白进行进一步的优化。Protein A/G免疫磁珠是进行IP实验的最佳方案。

Figure 1 General Protocol for Immunoprecipitation

表1 免疫共沉淀（IP）的操作方法

表2. 推荐的缓冲液配方
缓冲液	内容
Binding buffer	50 mM Tris, 150 mM NaCl, 0.1%-0.5% Triton 100 or Tween 20, pH 7.5
Wash buffer	50 mM Tris, 150 mM NaCl, 0.1%-0.5% Triton 100 or Tween 20, pH 7.5
Elution buffer	Elution buffer: 0.1 M -0.2 M Glycine, 0.1%-0.5% Triton 100 or Tween 20, pH 2.5-3.1 (or 0.1 M citric acid, 0.1%-0.5% Triton 100 or Tween 20, pH 2.5-3.1 or 2.5 % Acetic Acid, 0.1 M -0.2 M Glycine, 0.1%-0.5% Triton 100 or Tween 20.)

实验之前的注意事项

1 进行IP实验之前，请仔细阅读操作说明；
2 该产品需要使用磁力架
3 使用之前，请将Protein A/G免疫磁珠混匀；
4 Protein A/G免疫磁珠应该保持在储存液中避免干掉；
5 Protein A/G免疫磁珠应避免冻融或离心；
6 为保证最佳结果，请选择特异性好的抗体；
7 根据不同的抗体和抗原仅进行实验方法的优化；

表1. 抗体与Protein A/G及Protein A的结合能力比较
Species	Antibody class	sProtein A/G	sProtein A
Human	Total IgG	＋＋＋＋＋	＋＋＋＋＋
	IgG1, IgG2	＋＋＋＋＋	＋＋＋＋＋
	IgG3	＋＋＋＋＋	＋
	IgG4	＋＋＋＋＋	＋＋＋＋＋
	IgM	＋	＋
	IgD	－	－
	IgA	＋	＋
	IgA1, IgA2	＋	＋
	IgE	＋＋＋	＋＋＋
	Fab	＋	＋
	ScFv	＋	＋
Mouse	Total IgG	＋＋＋＋＋	＋＋＋＋＋
	IgM	－	－
	IgG1	＋＋＋	＋
	IgG2a	＋＋＋	＋＋＋
	IgG2b	＋＋＋	＋＋＋
	IgG3	＋＋＋	＋＋＋＋＋
Rat	Total IgG	＋＋＋	＋
	IgG 1	＋＋＋	＋＋＋
	IgG2a	＋＋＋＋＋	＋＋＋
	IgG2b	＋	＋＋＋
	IgG2c	＋＋＋＋＋	＋＋＋
Cow	Total IgG	＋＋＋＋＋	＋
	IgG1	＋＋＋＋＋	＋
	IgG2	＋＋＋＋＋	＋＋＋＋＋
Goat	Total IgG	＋＋＋＋＋	＋
	IgG1	＋＋＋＋＋	＋
	IgG2	＋＋＋＋＋	＋＋＋＋＋
Sheep	Total IgG	＋＋＋＋＋	＋
	IgG1	＋＋＋＋＋	＋
	IgG2	＋＋＋＋＋	＋＋＋＋＋
Horse	Total IgG	＋＋＋＋＋	＋
	IgG(ab), IgG(c)	＋	－
	IgG(T)	＋＋＋＋＋	＋
Rabbit	Total IgG	＋＋＋＋＋	＋＋＋＋＋
Guinea pig	Total IgG	＋＋＋＋＋	＋＋＋＋＋
Hamster	Total IgG	＋＋＋	＋＋＋
Pig	Total IgG	＋＋＋＋＋	＋＋＋＋＋
Donkey	Total IgG	＋＋＋＋＋	＋＋＋
Cat	Total IgG	＋＋＋＋＋	＋＋＋＋＋
Dog	Total IgG	＋＋＋＋＋	＋＋＋＋＋
Monkey	Total IgG	＋＋＋＋＋	＋＋＋＋＋
Chicken	Total IgG	－	－